Part:BBa_K4825002:Design

ERG20ww

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 222
  Illegal BamHI site found at 1059
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 694
  

Design Notes

Geranyl pyrophosphate(GPP) is precursor of pinene, which is our desired product. Therefore a higher yield of GPP is desirable, while the basic level of FPP is crucial for yeast survival. A method is designed to increase production of GPP while keeping FPP at a low level. It is to introduce 2 mutation F96W and N127W in ERG20, creating ERG20ww. The mutation in ERG20ww enables the reduction in FPP synthesis(from GPP), while GPP synthesis is not influenced. As a result, less GPP will be transformed to FPP, and a higher yield of pinene could be achieved.

Source

Saccharomyces cerevisiae

References

CHEN Tianhua, ZHANG Ruosi, JIANG Guozhen, YAO Mingdong, LIU Hong, WANG Ying, XIAO Wenhai, YUAN Yingjin. Metabolic engineering of Saccharomyces cerevisiae for pinene production[J]. CIESC Journal, 2019, 70(1): 179-188