Coding

Part:BBa_K4825015

Designed by: Ye Siyi   Group: iGEM23_GreatBay-SCIE   (2023-10-11)
Revision as of 13:12, 11 October 2023 by Registry (Talk | contribs)

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ARO10

ARO10 is derived from S.cerevisiae genome. It encodes for a 2-ketoacid decarboxylase which catalyses the reaction from 2-ketoacid to an aldehyde. It is included in our composite part with ADH7 (alcohol dehydrogenase) and ATF1 (alcohol acetyltransferase), which are transformed into the genome of S.cerevisiae simultaneously for overexpression to produce isoamyl acetate.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1791
    Illegal SpeI site found at 742
    Illegal PstI site found at 620
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1791
    Illegal SpeI site found at 742
    Illegal PstI site found at 620
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1791
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1791
    Illegal SpeI site found at 742
    Illegal PstI site found at 620
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1791
    Illegal SpeI site found at 742
    Illegal PstI site found at 620
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None