Coding

Part:BBa_K4613216

Designed by: Qinfei Zhou   Group: iGEM23_NAU-CHINA   (2023-10-09)
Revision as of 18:16, 9 October 2023 by QinfeiZhou (Talk | contribs)


traI

The tra system in Agrobacterium tumefaciens is a LuxR/LuxI-type quorum sensing system. TraI protein facilitates the formation of ligand 3-oxo-C8-HSL(3OC8), and TraR, when bound to 3OC8, becomes active and combines to Ptra, increasing its transcription efficiency.

Reference

  1. Fuqua WC, Winans SC. A LuxR-LuxI type regulatory system activates Agrobacterium Ti plasmid conjugal transfer in the presence of a plant tumor metabolite. J Bacteriol. 1994 May;176(10):2796-806. doi: 10.1128/jb.176.10.2796-2806.1994. PMID: 8188582; PMCID: PMC205432.
  2. White CE, Winans SC. The quorum-sensing transcription factor TraR decodes its DNA binding site by direct contacts with DNA bases and by detection of DNA flexibility. Mol Microbiol. 2007 Apr;64(1):245-56. doi: 10.1111/j.1365-2958.2007.05647.x. PMID: 17376086.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 401
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None