Coding

Part:BBa_K4613216

Designed by: Qinfei Zhou   Group: iGEM23_NAU-CHINA   (2023-10-09)


traI

The tra system in Agrobacterium tumefaciens is a LuxR/LuxI-type quorum sensing system. TraI protein facilitates the formation of ligand 3-oxo-C8-HSL(3OC8), and TraR, when bound to 3OC8, becomes active and combines to Ptra, increasing its transcription efficiency.

Reference

  1. Fuqua C, Winans S C.Conserved cis-acting promoter elements are required for density-dependent transcription of Agrobacterium tumefaciens conjugal transfer genes[J].J Bacteriol,1996, 178 (2): 435-40.
  2. White C E, Winans S C.The quorum-sensing transcription factor TraR decodes its DNA binding site by direct contacts with DNA bases and by detection of DNA flexibility[J].Mol Microbiol,2007, 64 (1): 245-56.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 401
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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