Composite

Part:BBa_K4585010

Designed by: Zijun Zhang   Group: iGEM23_CSU-CHINA   (2023-10-04)
Revision as of 03:04, 5 October 2023 by Registry (Talk | contribs)

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pGL4.35-3xHA-9xGAL4UAS-KRAB-Degron-NLS linearized vector

The purpose of this linear vector is for subsequent homologous recombination synthesis of the pGL4.35-3 HA-9 GAL4UAS-KRAB-Degron-NLS plasmid .

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4433
    Illegal XbaI site found at 161
    Illegal XbaI site found at 1943
    Illegal XbaI site found at 4270
    Illegal XbaI site found at 4505
    Illegal SpeI site found at 4029
    Illegal PstI site found at 3118
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4433
    Illegal NheI site found at 4461
    Illegal SpeI site found at 4029
    Illegal PstI site found at 3118
    Illegal NotI site found at 3097
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4433
    Illegal BglII site found at 4508
    Illegal BamHI site found at 423
    Illegal BamHI site found at 5100
    Illegal BamHI site found at 5325
    Illegal XhoI site found at 4313
    Illegal XhoI site found at 4870
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4433
    Illegal XbaI site found at 161
    Illegal XbaI site found at 1943
    Illegal XbaI site found at 4270
    Illegal XbaI site found at 4505
    Illegal SpeI site found at 4029
    Illegal PstI site found at 3118
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4433
    Illegal XbaI site found at 161
    Illegal XbaI site found at 1943
    Illegal XbaI site found at 4270
    Illegal XbaI site found at 4505
    Illegal SpeI site found at 4029
    Illegal PstI site found at 3118
    Illegal NgoMIV site found at 65
    Illegal NgoMIV site found at 176
    Illegal NgoMIV site found at 1654
    Illegal NgoMIV site found at 1671
    Illegal NgoMIV site found at 1803
    Illegal NgoMIV site found at 1894
    Illegal NgoMIV site found at 2146
    Illegal AgeI site found at 1949
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4789
    Illegal SapI site found at 444
    Illegal SapI site found at 2155
    Illegal SapI.rc site found at 5283


[edit]
Categories
Parameters
None