Part:BBa_K4585010

pGL4.35-3xHA-9xGAL4UAS-KRAB-Degron-NLS linearized vector

The purpose of this linear vector is for subsequent homologous recombination synthesis of the pGL4.35-3×HA-9×GAL4UAS-KRAB-Degron-NLS plasmid.

pGL4.35-3×HA-9×GAL4UAS-KRAB-Degron-NLS linearized vector

We based on the sequence of the pGL4.35-3×HA-9×GAL4UAS-KRAB-NLS plasmid. Special primers were designed to cut the plasmid into a linear sequence of length of about 5338 bp by PCR. The two ends of this linear sequence can be complementary to the Degron homologous recombination fragment of the homologous Degron fragment for homologous recombination to obtain the target product pGL4.35-3×HA-9×GAL4UAS-KRAB-Degron-NLS plasmid.

1 Diagrams

Fig.1 The agarose gel electrophoresis of pGL4.35-3×HA-9×GAL4UAS-KRAB-Degron-NLS linearized vector

2 Caution

The two ends of the pGL4.35-3×HA-9×GAL4UAS-KRAB-Degron-NLS linearized vectror need to be complementary to the two ends of the corresponding recombinant fragments to ensure the success of homologous recombination ligation.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 4433
  Illegal XbaI site found at 161
  Illegal XbaI site found at 1943
  Illegal XbaI site found at 4270
  Illegal XbaI site found at 4505
  Illegal SpeI site found at 4029
  Illegal PstI site found at 3118
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 4433
  Illegal NheI site found at 4461
  Illegal SpeI site found at 4029
  Illegal PstI site found at 3118
  Illegal NotI site found at 3097
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 4433
  Illegal BglII site found at 4508
  Illegal BamHI site found at 423
  Illegal BamHI site found at 5100
  Illegal BamHI site found at 5325
  Illegal XhoI site found at 4313
  Illegal XhoI site found at 4870
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 4433
  Illegal XbaI site found at 161
  Illegal XbaI site found at 1943
  Illegal XbaI site found at 4270
  Illegal XbaI site found at 4505
  Illegal SpeI site found at 4029
  Illegal PstI site found at 3118
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 4433
  Illegal XbaI site found at 161
  Illegal XbaI site found at 1943
  Illegal XbaI site found at 4270
  Illegal XbaI site found at 4505
  Illegal SpeI site found at 4029
  Illegal PstI site found at 3118
  Illegal NgoMIV site found at 65
  Illegal NgoMIV site found at 176
  Illegal NgoMIV site found at 1654
  Illegal NgoMIV site found at 1671
  Illegal NgoMIV site found at 1803
  Illegal NgoMIV site found at 1894
  Illegal NgoMIV site found at 2146
  Illegal AgeI site found at 1949
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 4789
  Illegal SapI site found at 444
  Illegal SapI site found at 2155
  Illegal SapI.rc site found at 5283