Primer

Part:BBa_K4790009

Designed by: Hongyan Jing   Group: iGEM23_NNU-CHINA   (2023-08-14)
Revision as of 13:38, 14 August 2023 by Registry (Talk | contribs)

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ASR1-F213S-F

The primer was designed to perform site-directed mutation which was defined as a forward primer. It can mutate base sequence which is on code 59 and coding the 213th amino acid from phenylalanine to serine. According to Codon preference in E.coli, AGC has the highest possibility coding serine.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None