Primer

Part:BBa_K4790009

Designed by: Hongyan Jing   Group: iGEM23_NNU-CHINA   (2023-08-14)


ASR1-F213S-F

The primer was designed to perform site-directed mutation which was defined as a forward primer. It can mutate base sequence which is on node 59 and coding the 213th amino acid from phenylalanine to serine. According to Codon preference in E.coli, AGC has the highest possibility coding serine.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None