Coding

Part:BBa_K4765008

Designed by: Siliang Zhan   Group: iGEM23_Fudan   (2023-09-30)
Revision as of 06:36, 2 October 2023 by YijunWang (Talk | contribs) (→‎Usage and Biology)


INPNC

contributed by Fudan iGEM 2023

Introduction

INPNC is a kind of modified INP with truncation of the entire internal repeating domain. INPNC does not appear to be hampered by the size of the passenger while also exhibiting compatibility with the translocation and surface display of proteins containing multiple cofactors and disulfide bond-containing passengers [1].

Usage and Biology

At the C-terminus of INPNC+linker, specific functional proteins can be attached to enable the surface display of it. In our project, we constructed BBa_K4765107 (ribozyme+strong RBS+INPNC-Ag3 fusion+stem-loop) and K4765108 ribozyme+strong RBS+INPNC-Nb3 fusion+stem-loop for the surface display of Ag/Nb3 and compared it with K4765105 ribozyme+strong RBS+intimin-Ag3 fusion+stem-loop, K4765106 ribozyme+strong RBS+intimin-Nb3 fusion+stem-loop, and selected intimin as our surface display system.

Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 481
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 330
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 72
    Illegal NgoMIV site found at 405
    Illegal AgeI site found at 429
  • 1000
    COMPATIBLE WITH RFC[1000]


Reference

  1. ↑ van Bloois, E., Winter, R. T., Kolmar, H., & Fraaije, M. W. (2011). Decorating microbes: Surface display of proteins on Escherichia coli. Trends in Biotechnology, 29(2), 79–86. https://doi.org/10.1016/j.tibtech.2010.11.003
[edit]
Categories
//cds/membrane
Parameters
protein