Part:BBa_K4765107
Twister P1 + T7_RBS + INPNC-Ag3 fusion + stem-loop
Contents
introduction
INPNC-Ag3 fusion is composed of a surface display system (INPNC+linker) and the coding sequence of a nanobody. INPNC exhibits compatibility with the translocation and surface display of proteins containing multiple cofactors and disulfide bond-containing passengers[1].Ag3 is a corresponding antigen of BBa_K4765007(Nb3)[2]. The interaction between Ag-Nb can mediate specific adhesion of Escherichia coli. A flexible protein domain linker of 10 aa was introduced between INPNC and Ag3 to ensure independent functionality of Ag3 and INPNC with minimal mutual disruption.
Usage and Biology
The surface-displayed antigen can specifically interact with the nanobody produced by BBa_K4765108.
Characterization
Sequencing map
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| Figure 1. Sequencing map of INPNC-Ag3 fusion
Sequencing is performed using the primer：Kan-F: 5-ATTCTCACCGGATTCAGT-3. |
Selection through Aggregation Assay
Get details in BBa_K4765108.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 542
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 391
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 133
Illegal NgoMIV site found at 466
Illegal AgeI site found at 490 - 1000COMPATIBLE WITH RFC[1000]
Reference
- ↑ van Bloois, E., Winter, R. T., Kolmar, H., & Fraaije, M. W. (2011). Decorating microbes: Surface display of proteins on Escherichia coli. Trends in Biotechnology, 29(2), 79–86. https://doi.org/10.1016/j.tibtech.2010.11.003
- ↑ Glass, D. S., & Riedel-Kruse, I. H. (2018). A Synthetic Bacterial Cell-Cell Adhesion Toolbox for Programming Multicellular Morphologies and Patterns. Cell, 174(3), 649-658.e16. https://doi.org/10.1016/j.cell.2018.06.041
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