Coding

Part:BBa_K4891007:Design

Designed by: Kexin Fei   Group: iGEM23_Guangzhou-MedX   (2023-06-27)
Revision as of 02:42, 28 June 2023 by Registry (Talk | contribs)

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aroL


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 425
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 425
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 215
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 425
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 425
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

These recombinant plasmids serve as homologous fragments for knocking out the target genes by CRISPR-Cas9.


Source

AroK and AroL (encoding shikimic acid kinase), PtsH and PtsI (encoding phosphotransferase system) are obtained from the genome of E. coli MG1655.

References