Coding
Part:BBa_K4891007:Design
Designed by: Kexin Fei Group: iGEM23_Guangzhou-MedX (2023-06-27)
aroL
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 425
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 425
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 215
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 425
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 425
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
These recombinant plasmids pBAD33-aroL serves as homologous fragments and guide RNA for knocking out the target genes by CRISPR-Cas9, respectively.
Source
AroK and AroL (encoding shikimic acid kinase) are obtained from the genome of E. coli MG1655. In addition, recombinant plasmids pTargetF-sgRNAaroK/aroL initiate with pTargetF, which stores in our lab.