Primer

Part:BBa_K4790044

Designed by: Yi Zheng   Group: iGEM23_NNU-CHINA   (2023-08-14)
Revision as of 06:42, 14 August 2023 by Registry (Talk | contribs)

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ASR1-Y46I-F

The primer was designed to perform site-directed mutation which was defined as a forward primer. It can mutate base sequence which is on code 59 and coding the 46th amino acid from tyrosine to isoleucine. According to Codon preference in E.coli, ATT has the highest possibility coding alanine.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None