Primer

Part:BBa_K4790044

Designed by: Yi Zheng   Group: iGEM23_NNU-CHINA   (2023-08-14)


ASR1-Y46I-F

The primer was designed to perform site-directed mutation which was defined as a forward primer. It can mutate base sequence which is on node 59 and coding the 46th amino acid from tyrosine to isoleucine. According to Codon preference in E.coli, ATT has the highest possibility coding isoleucine.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None