Plasmid

Part:BBa_J435114

Designed by: Drew Endy, Keith Shearwin   Group: FreeGenes   (2023-05-23)
Revision as of 16:14, 23 May 2023 by Registry (Talk | contribs)

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pE-FLP (AmpR Low-copy)

Can be used to remove the integration module (including the antibiotic resistance cassette) from pOSIP integrants.

One-Step Cloning and Chromosomal Integration of DNA

https://freegenes.github.io/genes/BBF10K_000016.html


One-step cloning and chromosomal integration of DNA. St-Pierre F, Cui L, Priest DG, Endy D, Dodd IB, Shearwin KE. ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20. 10.1021/sb400021j PubMed 24050148

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 837
    Illegal SpeI site found at 1155
    Illegal SpeI site found at 2573
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 837
    Illegal SpeI site found at 1155
    Illegal SpeI site found at 2573
    Illegal NotI site found at 2892
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 837
    Illegal BglII site found at 25
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 837
    Illegal SpeI site found at 1155
    Illegal SpeI site found at 2573
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 837
    Illegal SpeI site found at 1155
    Illegal SpeI site found at 2573
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3538


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