Part:BBa_J435114

pE-FLP (AmpR Low-copy)

Can be used to remove the integration module (including the antibiotic resistance cassette) from pOSIP integrants.

One-Step Cloning and Chromosomal Integration of DNA

For propagation, please grow this plasmid in the original strain (E811) In that strain, the strong promoter pE will be repressed, thus avoiding possible toxicity and accumulation of unwanted mutations in the plasmid. Use 50ug/ml ampicillin (100ug/ml may inhibit growth)

https://freegenes.github.io/genes/BBF10K_000016.html

One-step cloning and chromosomal integration of DNA. St-Pierre F, Cui L, Priest DG, Endy D, Dodd IB, Shearwin KE. ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20. 10.1021/sb400021j PubMed 24050148

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 837
  Illegal SpeI site found at 1155
  Illegal SpeI site found at 2573
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 837
  Illegal SpeI site found at 1155
  Illegal SpeI site found at 2573
  Illegal NotI site found at 2892
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 837
  Illegal BglII site found at 25
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 837
  Illegal SpeI site found at 1155
  Illegal SpeI site found at 2573
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 837
  Illegal SpeI site found at 1155
  Illegal SpeI site found at 2573
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 3538