Coding

Part:BBa_K4260030

Designed by: Claudia Angélica García Alonso   Group: iGEM22_TecCEM   (2022-10-13)
Revision as of 00:07, 14 October 2022 by Registry (Talk | contribs)

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IemR: Transcriptional activator of Iso

This part codes a transcriptional activator, specific for the transcription of the coding gene of Isoeugenol monooxygenase, an enzyme that helps the direct conversion of isoeugenol to vanillin. It has been proven that the expression of this gene, signinficantly increases the expression of Isouegenol monooxygenase (Ryu, J. Y., Seo, J., Ahn, J. H., Sadowsky, M. J., & Hur, H. G. (2012). Transcriptional control of the isoeugenol monooxygenase of Pseudomonas nitroreducens Jin1 in Escherichia coli. Bioscience, biotechnology, and biochemistry, 76(10), 1891-1896)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 516
    Illegal XhoI site found at 661
    Illegal XhoI site found at 688
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None