Coding
Part:BBa_K4260030
Designed by: Claudia Angélica García Alonso Group: iGEM22_TecCEM (2022-10-13)
IemR: Transcriptional activator of Iso
This part codes a transcriptional activator, specific for the transcription of the coding gene of Isoeugenol monooxygenase, an enzyme that helps the direct conversion of isoeugenol to vanillin. It has been proven that the expression of this gene, signinficantly increases the expression of Isouegenol monooxygenase (Ryu, J. Y., Seo, J., Ahn, J. H., Sadowsky, M. J., & Hur, H. G. (2012). Transcriptional control of the isoeugenol monooxygenase of Pseudomonas nitroreducens Jin1 in Escherichia coli. Bioscience, biotechnology, and biochemistry, 76(10), 1891-1896)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 516
Illegal XhoI site found at 661
Illegal XhoI site found at 688 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |