Part:BBa_K4260030
IemR: Transcriptional activator of Iso
Type:Basic part
Designed by:Claudia Angélica GarcÃa Alonso
Group:iGEM_TecCEM
This part codes a transcriptional activator, specific for the transcription of the coding gene of Isoeugenol monooxygenase, an enzyme that helps the direct conversion of isoeugenol to vanillin. It has been proven that the expression of this gene, significantly increases the expression of Isoeugenol monooxygenase (Ryu, J. Y., Seo, J., Ahn, J. H., Sadowsky, M. J., & Hur, H. G. (2012). Transcriptional control of the isoeugenol monooxygenase of Pseudomonas nitroreducens Jin1 in Escherichia coli. Bioscience, biotechnology, and biochemistry, 76(10), 1891-1896) .
Usage and Biology
In the picture (fig 3), it is seen that in the middle of the structure, the value of the potential field is mostly negative (red), whilst the exterior of the molecule appears to be blue which means that the protein would present a higher affinity with some other target molecule. Nevertheless, the charge that domains in this molecule are neutral (white).
In the picture, it is seen that mostly in the center of the molecular protein has more non-polar activity, whether the value has bigger polar residues the activity increases its neutral activity then its positive polar activity, so the extremes of the protein have more positive polar activity.
Application
For more information please check https://parts.igem.org/Part:BBa_K4260006 .
Biosafety
Although this coding sequence comes from a Pseudomonas bacteria, it is not associated with the pathogenicity of the microorganism itself.
References
[1] Ryu, J. Y., Seo, J., Ahn, J. H., Sadowsky, M. J., & Hur, H. G. (2012). Transcriptional control of the isoeugenol monooxygenase of Pseudomonas nitroreducens Jin1 in Escherichia coli. Bioscience, biotechnology, and biochemistry, 76(10), 1891-1896.
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