Regulatory

Part:BBa_K4317014

Designed by: Chris Song   Group: iGEM22_Seoul_Korea   (2022-10-12)
Revision as of 13:04, 12 October 2022 by Envien mc (Talk | contribs)


PtrnQ

trnQ codes for arginine-specific tRNA from Basillus subtilis

800px-TrnQ_ref.jpeg.png

PtrnQ was only twice as high as that from the P43 promoter.

800px-PtrnQ.png

Reference

Song, Yafeng, et al. "Promoter screening from Bacillus subtilis in various conditions hunting for synthetic biology and industrial applications." PloS one 11.7 (2016): e0158447.

Usage and Biology

800px-Promoter_strength.png

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 7
    Illegal XbaI site found at 16
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 7
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 7
    Illegal BglII site found at 13
    Illegal BamHI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 7
    Illegal XbaI site found at 16
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 7
    Illegal XbaI site found at 16
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None