Regulatory

Part:BBa_K4317014

Designed by: Chris Song   Group: iGEM22_Seoul_Korea   (2022-10-12)


PtrnQ

trnQ codes for arginine-specific tRNA from Basillus subtilis

800px-TrnQ_ref.jpeg.png

PtrnQ was only twice as high as that from the P43 promoter.

800px-PtrnQ.png


Usage and Biology

800px-Promoter_strength.png

We registered 5 promoters, 5 secretion signals, and 4 cellulose degrading enzymes as new basic parts. Our newly registered P2069M (BBa_K4317001) and PtrnQ (BBa_K4317004) constitutive promoters can be used in both Bacillus and E. coli. In particular, in Bacillus, these promoters showed higher transcriptional activity than the previously known P43 promoter.

Reference

Song, Yafeng, et al. "Promoter screening from Bacillus subtilis in various conditions hunting for synthetic biology and industrial applications." PloS one 11.7 (2016): e0158447.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 7
    Illegal XbaI site found at 16
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 7
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 7
    Illegal BglII site found at 13
    Illegal BamHI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 7
    Illegal XbaI site found at 16
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 7
    Illegal XbaI site found at 16
  • 1000
    COMPATIBLE WITH RFC[1000]


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