Regulatory
Part:BBa_K4317014
Designed by: Chris Song Group: iGEM22_Seoul_Korea (2022-10-12)
PtrnQ
trnQ codes for arginine-specific tRNA from Basillus subtilis
PtrnQ was only twice as high as that from the P43 promoter.
Usage and Biology
We registered 5 promoters, 5 secretion signals, and 4 cellulose degrading enzymes as new basic parts. Our newly registered P2069M (BBa_K4317001) and PtrnQ (BBa_K4317004) constitutive promoters can be used in both Bacillus and E. coli. In particular, in Bacillus, these promoters showed higher transcriptional activity than the previously known P43 promoter.
Reference
Song, Yafeng, et al. "Promoter screening from Bacillus subtilis in various conditions hunting for synthetic biology and industrial applications." PloS one 11.7 (2016): e0158447.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 7
Illegal XbaI site found at 16 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 7
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 7
Illegal BglII site found at 13
Illegal BamHI site found at 1 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 7
Illegal XbaI site found at 16 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 7
Illegal XbaI site found at 16 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |