Composite
Part:BBa_K4206003
Designed by: Kazuma Tsurumaki Group: iGEM22_Qdai (2022-10-04)
cellulases with PhoA signal sequence
This part contains the coding sequences of endo-1,4-beta-glucanases, exo-1,4-beta-glucanases and beta-glucosidases with PhoA signal sequence. The complete cellulose hydrolysis reaction proceeds by these enzymes.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 4670
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 4303
Illegal NgoMIV site found at 4676
Illegal NgoMIV site found at 5178
Illegal AgeI site found at 331
Illegal AgeI site found at 1795 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 4723
Illegal SapI.rc site found at 4806
[edit]
Categories
Parameters
None |