Composite

Part:BBa_K4206003

Designed by: Kazuma Tsurumaki   Group: iGEM22_Qdai   (2022-10-04)


cellulases with PhoA signal sequence

This part contains the coding sequences of endo-1,4-beta-glucanases, exo-1,4-beta-glucanases and beta-glucosidases with PhoA signal sequence. All sequences were optimized for E.coli. The complete cellulose hydrolysis reaction proceeds by these enzymes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 4670
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4303
    Illegal NgoMIV site found at 4676
    Illegal NgoMIV site found at 5178
    Illegal AgeI site found at 331
    Illegal AgeI site found at 1795
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4723
    Illegal SapI.rc site found at 4806


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