Composite
Part:BBa_K4256177
Designed by: Jingke Wang Group: iGEM22_UCAS-China (2022-09-29)
FNR expression system
The expression product of the BBa_K4256177 system, FNR, is a sensing protein containing the [Fe-S] cluster that acts as a transcriptional regulator to regulate gene expression downstream of the oxygen-controlled promoter HIP-1.Under hypoxic conditions, the [4Fe-4S] cluster, which is stably present in the FNR protein, can prompt the FNR protein to form a homodimer, and this dimer can specifically bind to DNA sites and initiate ccdA expression downstream of the target promoter HIF-1; under normoxic conditions, the [4Fe-4S] cluster is disassembled, and the FNR dimer is depolymerized, so the initiation of ccdA transcription is repressed.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 651
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 651
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 651
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 651
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 651
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |