Composite

Part:BBa_K4256177

Designed by: Jingke Wang   Group: iGEM22_UCAS-China   (2022-09-29)


FNR expression system

The expression product of the BBa_K4256177 system, FNR, is a sensing protein containing the [Fe-S] cluster that acts as a transcriptional regulator to regulate gene expression downstream of the oxygen-controlled promoter HIP-1.Under hypoxic conditions, the [4Fe-4S] cluster, which is stably present in the FNR protein, can prompt the FNR protein to form a homodimer, and this dimer can specifically bind to DNA sites and initiate ccdA expression downstream of the target promoter HIP-1; under normoxic conditions, the [4Fe-4S] cluster is disassembled, and the FNR dimer is depolymerized, so the initiation of ccdA transcription is repressed.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 651
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 651
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 651
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 651
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 651
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None