Composite

Part:BBa_K4156111

Designed by: Zheng Huang   Group: iGEM22_LZU-CHINA   (2022-10-07)
Revision as of 06:53, 9 October 2022 by Liwei (Talk | contribs)


pCadC-mRFP

pCaCd-mRFP is a biological brick that expresses mRFP under the control of the pCaCd promoter (BBa_K4156076). pCaCd-mRFP functions as a red fluorescent signal to characterize the performance of the pCaCd promoter.


Usage and Biology

In this biobrick, we used the pCaCd promoter to regulate downstream mRFP expression and eventually introduced the rrnB T1 and T7Te dual terminators. in addition, we added the RiboJ sequence and RBS B0034 for optimization. This design allowed the spatiotemporal intensity of gene expression under the regulation of the pCaCd promoter to be measured by the red fluorescent signal. We were thus able to verify the specific response of the pCaCd promoter to pH changes.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 731
    Illegal AgeI site found at 843
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None