Part:BBa_K4156111
pCadC-mRFP
pCadC-mRFP is a biological brick that expresses mRFP under the control of the pCadC promoter ( BBa_K4156076 ). pCadC-mRFP functions as a red fluorescent signal to characterize the performance of the pCaCd promoter.
Usage and Biology
In this biobrick, we used the pCadC promoter to regulate downstream mRFP expression and eventually introduced the rrnB T1 and T7Te dual terminators. in addition, we added the RiboJ sequence and RBS B0034 for optimization. This design allowed the spatiotemporal intensity of gene expression under the regulation of the pCaCd promoter to be measured by the red fluorescent signal. We were thus able to verify the specific response of the pCaCd promoter to pH changes.
Characterization
pH induced promoter testing
We constructed a pH reporter consisting of the pH-inducible promoter pCadC+mRFP. To test itsperformance, we added reporter in different chassis organisms. Fig 1 illustrates that pCadC induces the expression of the downstream gene mRFP with the decrease of pH,. Thus, it can be seen that pH reporter can work properly.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 731
Illegal AgeI site found at 843 - 1000COMPATIBLE WITH RFC[1000]
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