Part:BBa_K4143340

AMP Encapsulin Composite

This composite part includes an antimicrobial peptide (HBCM2), TEV protease site, targeting peptide, and T4GALA encapsulin. The encapsulin is a protein nanocompartment that sequesters cargo carrying a targeting peptide. In this case, the antimicrobial peptide (AMP) is the cargo and it's attached to the targeting peptide to be sequestered within the encapsulin. This serves to protect the bacterial host from the AMP that it's producing [1]. The TEV protease site attaches the targeting peptide and AMP so the AMP can be isolated later on in downstream purification. This was our primary experimental construct that was compared against two controls: one with just the AMP (positive control) and one with just the encapsulin (negative control). We evaluated the encapsulin’s ability to sequester the AMP through several in-lab assays and mathematical modeling.

Figure 1: Experimental construct

Usage and Biology

Molecular Dynamics of AMP-Encapsulin Stability

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 191
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]