User contributions
- 15:05, 11 October 2022 (diff | hist) . . (+3) . . Part:BBa K4143340 (current)
- 15:04, 11 October 2022 (diff | hist) . . (-1) . . Part:BBa K4143337 (current)
- 15:03, 11 October 2022 (diff | hist) . . (-1) . . Part:BBa K4143336 (current)
- 15:02, 11 October 2022 (diff | hist) . . (+419) . . N File:Results-4-updated.png (SDS-PAGE gel showing heat protein purification. Note lanes 4, 6, and 7 showing the increasing purity of samples heated to 25, 65, and 85 degrees, respectively. From left: ladder, clear lysate using dilute lysozyme, clear lysate using moderate lysozyme,...) (current)
- 15:01, 11 October 2022 (diff | hist) . . (+260) . . N File:Results-5-updated.png (SDS-PAGE gel demonstrating ammonium sulfate purification. From left: lanes show protein pellets collected after incubating with ammonium sulfate at concentrations ranging from 10% to 100% in 10% intervals. Note the high purity of the lower concentratio...) (current)
- 23:54, 9 October 2022 (diff | hist) . . (+3) . . Part:BBa K4143336
- 22:31, 9 October 2022 (diff | hist) . . (+1) . . Part:BBa K4143340
- 21:48, 9 October 2022 (diff | hist) . . (+1) . . Part:BBa K4143340
- 21:47, 9 October 2022 (diff | hist) . . (+286) . . N File:Growth-results.png (Growth assay average growth rates for 4 experimental groups: empty pET vector, encapsulin only, AMP only, and encapsulin + AMP (this part). Error bars represent 95% confidence intervals. A) Growth rates for uninduced gene expression B) Growth rates for...) (current)
- 21:46, 9 October 2022 (diff | hist) . . (-42) . . Part:BBa K4143340
- 21:43, 9 October 2022 (diff | hist) . . (+8,259) . . Part:BBa K4143340
- 21:38, 9 October 2022 (diff | hist) . . (+150) . . Part:BBa K4143340
- 21:36, 9 October 2022 (diff | hist) . . (+360) . . Part:BBa K4143340
- 21:32, 9 October 2022 (diff | hist) . . (+174) . . N File:AMP-encapsulin-interface.png (Visualization of the upright encapsulin-antimicrobial peptide complex system. Bending at the same residue as within the single-AMP system (Thr20-Thr21) are visible here, too.) (current)
- 21:31, 9 October 2022 (diff | hist) . . (+230) . . N File:AMP-encapsulin-MD-stability.png (RMSD and RMSF graphs of the upright and inverted systems. Overall, systems where the AMP is in close proximity to the encapsulin wall seem to have reduced structural change over time. Note the high RMSF between residues 38 and 48.) (current)
- 21:27, 9 October 2022 (diff | hist) . . (+433) . . N File:SDS-purification-1.png (SDS-PAGE gel showing protein purification. Note lanes 4, 6, and 7 from left showing the increasing purity of samples heated to 25, 65, and 85 degrees, respectively. From left, ladder, clear lysate using dilute lysozyme, clear lysate using moderate lyso...) (current)
- 21:23, 9 October 2022 (diff | hist) . . (0) . . N File:Growth-assay.png (current)
- 21:22, 9 October 2022 (diff | hist) . . (+70) . . N File:Math-model-results.png (Predicted kinetics of AMP and encapsulin production and self-assembly) (current)
- 21:20, 9 October 2022 (diff | hist) . . (+75) . . N File:Math-model.png (Figure 2. Mathematical model of encapsulin + AMP expression + encapsulation) (current)
- 19:56, 9 October 2022 (diff | hist) . . (+136) . . Part:BBa K4143339 (current)
- 19:55, 9 October 2022 (diff | hist) . . (+930) . . Part:BBa K4143339
- 19:46, 9 October 2022 (diff | hist) . . (0) . . Part:BBa K4143337
- 19:46, 9 October 2022 (diff | hist) . . (+1,588) . . Part:BBa K4143337
- 19:33, 9 October 2022 (diff | hist) . . (+89) . . N File:Enc-gel.png (Figure 1: Agarose gel electrophoresis of T4GALA encapsulin PCR product (shown in lane 5).) (current)
- 19:32, 9 October 2022 (diff | hist) . . (-61) . . Part:BBa K4143337
- 19:04, 9 October 2022 (diff | hist) . . (-75) . . Part:BBa K4143336
- 19:04, 9 October 2022 (diff | hist) . . (-69) . . Part:BBa K4143336
- 19:03, 9 October 2022 (diff | hist) . . (+451) . . Part:BBa K4143336
- 19:01, 9 October 2022 (diff | hist) . . (0) . . Part:BBa K4143336
- 19:01, 9 October 2022 (diff | hist) . . (+74) . . Part:BBa K4143336
- 18:59, 9 October 2022 (diff | hist) . . (+32) . . Part:BBa K4143336
- 18:58, 9 October 2022 (diff | hist) . . (+3,509) . . Part:BBa K4143336
- 18:40, 9 October 2022 (diff | hist) . . (+51) . . N File:SDS-purification-2.png (Figure 3: SDS-PAGE gel of AMP (HBCM2) + encapsulin.) (current)
- 18:38, 9 October 2022 (diff | hist) . . (+290) . . N File:HBCM2-modeling.png (Figure 2: Graphs of the root mean square fluctuation (RMSF) and root mean square deviation (RMSD) of the antimicrobial peptide. Given the low overall values (<2nm), it seems that this peptide is relatively stable in solvent. The RMSD graph is interpola...) (current)
- 18:36, 9 October 2022 (diff | hist) . . (+72) . . N File:HBCM2-alphafold.png (Figure 1: AlphaFold structural predictions for HBCM2 + targeting peptide) (current)
- 15:58, 9 October 2022 (diff | hist) . . (-436) . . Part:BBa K4143336 (→References)
- 20:53, 27 September 2022 (diff | hist) . . (0) . . Part:BBa K4143340
- 20:51, 27 September 2022 (diff | hist) . . (-83) . . Part:BBa K4143340
- 20:50, 27 September 2022 (diff | hist) . . (+43) . . Part:BBa K4143340
- 20:48, 27 September 2022 (diff | hist) . . (+20) . . N File:Experiment-construct.png (Experiment construct) (current)
- 20:44, 27 September 2022 (diff | hist) . . (+22) . . N File:AMP-encapsulin.png (Experimental Construct) (current)
- 17:28, 27 September 2022 (diff | hist) . . (0) . . Part:BBa K4143337
- 17:28, 27 September 2022 (diff | hist) . . (+195) . . Part:BBa K4143337
- 17:27, 27 September 2022 (diff | hist) . . (+51) . . Part:BBa K4143337
- 17:33, 21 September 2022 (diff | hist) . . (+7) . . Part:BBa K4143336
