Coding
Part:BBa_K4380011:Design
Designed by: Brigita Duchovska Group: iGEM22_Vilnius-Lithuania (2022-10-01)
eGFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 151
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 16
Design Notes
The design of this part used exploited type IIs restriction enzymes LguI ability to recognize asymmetric DNA sequences and cleave at a defined distance outside of its recognition sequence. When optimizing a vector sequence to use with this specific part, optimizations need to be perfomed so that LguI does not cleave DNA in any other spot, other than the plasmid and its insert.
Source
Synthetic