Coding

Part:BBa_K4214000

Designed by: Jol Tekoniemi   Group: iGEM22_Stockholm   (2022-10-07)
Revision as of 09:09, 9 October 2022 by Zeniazen5 (Talk | contribs)

Human Gastric Intrinsic Factor (hGIF)


This part codes for human Gastric Intrinsic Factor protein (AA19-417). The gene is located in chromosome 11q12.1. This gene is a part of cobalamin transporters group. It is essential for the absorption of vitamin B12. Mutations in this gene may lead to pernicious anemia.

We removed the native signal peptide (AA1-18) from the N-terminal in order to obtain a sequence that can be worked with more easily in bacterial systems. However, since we used bacterial and mammalian expression vectors in parallel, we needed to have compatible parts and decided to work only with part BBa_K4214001[1], which includes the signal peptide sequence (AA1-18).


File:File:Part-BBa K4214000.png
Fig.: Human GIF (BBa_K4214000



Usage and Biology

  • NCBI page of the human gastric intrinsic factor [2]
  • We designed this biobrick in order to have a sequence for our other parts
  • This part of hGIF should be mentioned that does not contain the signal peptide


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 265
    Illegal BsaI.rc site found at 945
    Illegal SapI site found at 935
[edit]
Categories
//cds/membrane/transporter
Parameters
None