![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K4165240
Designed by: Omnia AlaaEldeen Abdelazeem Group: iGEM22_CU_Egypt (2022-10-08)
His-UBC
his biobrick consists of T7 promotor (BBa_K3633015), RBS (BBa_K4165016), 6-His tag (BBa_K4165020), UBC (BBa_K4165014), T7 Terminator (BBa_K731721), The His tag was attached to the UBC coding sequence to serve in the purification using NI-NTA column.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 113
Illegal BamHI site found at 82 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |