Part:BBa_K4165240
His-UBC
his biobrick consists of T7 promotor (BBa_K3633015), RBS 1 (BBa_K4165261), 6-His tag (BBa_K4165020), UBC (BBa_K4165014), T7 Terminator (BBa_K731721), The His tag was attached to the UBC coding sequence to serve in the purification using NI-NTA column.
Usage and Biology
the ubiquitin C which is essential in the degradation of misfolded proteins through the ubiquitin-proteasome cascade. It has been used in our project as signaling for the degradation of both tau and Aβ proteins which are considered the main causes of Alzheimer’s Disease pathogenesis.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 113
Illegal BamHI site found at 82 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Dry lab
Mathematical modeling
Transcription rate and translation rate under T7 promotor
the mathematical modeling was based on our code for the calculation of transcription and translation (you can find it in the code section) beside with the estimated results from the wet lab.
Figure 1. this figure shows the results from the transcription and translation code showing the
the concentration of the protein with time.
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