Composite

Part:BBa_K4115046

Designed by: Kaijun Wang   Group: iGEM22_ShanghaiTech_China   (2022-09-30)
Revision as of 07:32, 4 October 2022 by Yurj (Talk | contribs)


NS3-2-KanR-J23101-B0034-LuxR-B0015-Lux pR-B0034-sfGFP-B0015-NS3-1

NS3-2-lac UV5 promoter-cscB-lacI-KanR-NS3-1
Function permease
Use in S.elongatus HL7942
RFC standard None
Backbone pUC57
Submitted by ShanghaiTech_China

This Composite part is integrated into the plasmid backbone of PUC57 and entered Synechococcus HL7942 by natural transformation with E. coli. LuxR is a common receptor protein in bacterial quorum sensing. The LuxR in this composite part plays a role in receiving and responding to AHL(3O C6 HSL) signal molecules [1]. When LuxR receives the signal of AHL, it can regulate the downstream Lux pR (BBa_R0062) to make superfolder GFP (BBa_K4115000) express.
We can construct a communication and regulation system between E.coli and S.elongatus HL7942
with Lux network.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 507
    Illegal PstI site found at 4261
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1167
    Illegal NheI site found at 1190
    Illegal PstI site found at 507
    Illegal PstI site found at 4261
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 507
    Illegal PstI site found at 4261
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 507
    Illegal PstI site found at 4261
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2145

References

[1] Kylilis, N., Tuza, Z.A., Stan, GB. et al. Tools for engineering coordinated system behaviour in synthetic microbial consortia. Nat Commun 9, 2677 (2018). https://doi.org/10.1038/s41467-018-05046-2

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