Coding

Part:BBa_K4115017:Design

Designed by: Shenxi Cao   Group: iGEM22_ShanghaiTech_China   (2022-09-23)
Revision as of 09:05, 3 October 2022 by ParadoxDecade (Talk | contribs) (→‎Design Notes)


SacC (the extracellular sucrase gene)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 437
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1309
    Illegal PstI site found at 437
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1352
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 437
  • 25
    INCOMPATIBLE WITH RFC[25]
    Unknown
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

SacC gene can enhance the ability of Escherichia coli to utilize environmental sucrose. Heterologous expression of SacC aims to improve the utilization of sucrose in the medium by E. coli responsible for the synthetic production of biologics.[1] At the same time, the introduction of SacC will cause E. coli to produce a large amount of organic acids, which is exactly the organic carbon source preferred by nitrogen-fixing bacteria (A.caul). The intersection of nutritional structures will help the entire symbiotic system to be more stable and efficient.

Source

The SacC gene was originally found in the Zymomonas mobilis genome. We obtain gene fragments through commercial synthesis companies.

References