Part:BBa_K4115017:Design
SacC (the extracellular sucrase gene)
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 437
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1352
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 437
Illegal NgoMIV site found at 696
Illegal AgeI site found at 418 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
SacC gene can enhance the ability of Escherichia coli to utilize environmental sucrose. Heterologous expression of SacC aims to improve the utilization of sucrose in the medium by E. coli responsible for the synthetic production of biologics.[1] At the same time, the introduction of SacC will cause E. coli to produce a large amount of organic acids, which is exactly the organic carbon source preferred by nitrogen-fixing bacteria (A.caul). The intersection of nutritional structures will help the entire symbiotic system to be more stable and efficient.
Source
The SacC gene was originally found in the Zymomonas mobilis genome. We obtain gene fragments through commercial synthesis companies.
References
[1] Lee, J.W., Choi, S., Park, J.H., Vickers, C.E., Nielsen, L.K., and Lee, S.Y. (2010). Development of sucrose-utilizing Escherichia coli K-12 strain by cloning beta-fructofuranosidases and its application for L-threonine production. Appl Microbiol Biotechnol 88, 905-913. 10.1007/s00253-010-2825-7.