Coding

Part:BBa_K4115017:Design

Designed by: Shenxi Cao   Group: iGEM22_ShanghaiTech_China   (2022-09-23)
Revision as of 08:45, 23 September 2022 by Registry (Talk | contribs)

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SacC (the extracellular sucrase gene)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 437
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1309
    Illegal PstI site found at 437
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1352
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 437
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 437
    Illegal NgoMIV site found at 696
    Illegal AgeI site found at 418
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

SacC gene can enhance the ability of Escherichia coli to utilize environmental sucrose.


Source

The SacC gene was originally found in the Zymomonas mobilis genome. We obtain gene fragments through commercial synthesis companies.

References