Composite

Part:BBa_K4408005

Designed by: Leyu Xu   Group: iGEM22_Worldshaper-NJBIOX   (2022-09-29)
Revision as of 10:07, 29 September 2022 by Registry (Talk | contribs)

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thl modified by oligopeptide SpyCatcher

This part is the coding region of thl enzyme combined with genes coding for SpyCatcher oligopeptide and linker 1. It is constructed by genes coding for thl(BBa_K3443003), SpyCatcher(BBa_K3329016) and linker 1(BBa_K4408003). It can be bounded with SpyTag-linker 1-hbd (BBa_K4408006) to enhance the production of butanol and butyl acid.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1076
    Illegal SapI.rc site found at 900


[edit]
Categories
Parameters
None