Composite

Part:BBa_K4408005

Designed by: Leyu Xu   Group: iGEM22_Worldshaper-NJBIOX   (2022-09-29)


thl modified by oligopeptide SpyCatcher

This part is the coding region of thl enzyme combined with genes coding for SpyCatcher oligopeptide and Linker 1. It is constructed by thl (BBa_K3443003), SpyCatcher (BBa_K3329016) and Linker 1(BBa_K1583012) sequences. It can be bounded with SpyTag-Linker 1-hbd (BBa_K4408006) to enhance the catalysis of acetyl-CoA into 3-hydroxybutytyl-CoA by thl and hbd enzymes in the metabolic pathway of Clostridium tyrobutyricum.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1076
    Illegal SapI.rc site found at 900


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Categories
Parameters
None