![](https://parts.igem.org/images/partbypart/icon_plasmid_backbone.png)
Plasmid_Backbone
Part:BBa_K4212029:Design
Designed by: Yuancheng Ding Group: iGEM22_Imperial_College_London (2022-09-30)
STK-1D-sfGFP
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 1102
Illegal XbaI site found at 1117
Illegal PstI site found at 2180 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 1102
Illegal PstI site found at 2180
Illegal NotI site found at 1108 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 1102 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 1102
Plasmid lacks a suffix.
Illegal PstI site found at 2180 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 1102
Plasmid lacks a suffix.
Illegal XbaI site found at 1117
Illegal PstI site found at 2180 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 2158
Illegal BsaI.rc site found at 1140
Illegal SapI.rc site found at 1324
Design Notes
sfGFP dropout cassette inserted within the MCS of backbone, flanking with BsaI and BsmBI sites. Dropout cassette is replaced by target sequences, which verifies the insertion of target sequences if transformants do not express sfGFP.
Source
Synthetic construct