Plasmid_Backbone

Part:BBa_K4212029:Design

Designed by: Yuancheng Ding   Group: iGEM22_Imperial_College_London   (2022-09-30)


STK-1D-sfGFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1102
    Illegal XbaI site found at 1117
    Illegal PstI site found at 2180
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1102
    Illegal PstI site found at 2180
    Illegal NotI site found at 1108
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1102
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 1102
    Plasmid lacks a suffix.
    Illegal PstI site found at 2180
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 1102
    Plasmid lacks a suffix.
    Illegal XbaI site found at 1117
    Illegal PstI site found at 2180
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 2158
    Illegal BsaI.rc site found at 1140
    Illegal SapI.rc site found at 1324


Design Notes

sfGFP dropout cassette inserted within the MCS of backbone, flanking with BsaI and BsmBI sites. The dropout cassette is replaced by target sequences, which verifies the insertion of target sequences if transformants do not express sfGFP.


Source

Synthetic construct

References