Reporter

Part:BBa_K3977002

Designed by: Yilong Xu   Group: iGEM21_SCU-China   (2021-10-01)
Revision as of 08:34, 26 September 2022 by Tigress K (Talk | contribs) (→‎Characterized by CAFA_China 2022)


mSarlet-I

mSarlet-I

Usage and Biology

Characterized by CAFA_China 2022

  • The mScarlet-I gene and the relE toxin gene were fused to construct a fusion gene, in order to detect the amount of relE protein synthesis. We removed the terminator of relE and fused it to the mScarlet-I gene.
  • We designed the genetic circuit that contains sulAp (sulA promoter), Amp30E, relE gene (RelE toxin), mScarlet-I and 3WJ-Bro(3WJ-Bro) , and transferred it into DH10B competent cells (The control group did not contain the Amp30E Amplification Device). The gene circuit diagram is shown bellow:
Gene circuit: pSB1C3-sulAp-Amp30E-relE-mScarlet-I-3WJ-Bro

  • After bacterial culture and UV induction, we measured the OD600 and fluorescence values under 579/616nm. Then, the fluorescence per OD was calculated.
  • The curve of experimental group was higher than that of control group as shown in the following figure, indicating the mScarlet-I correctly displayed the increasing tendency of relE protein synthesis under the effect of Amp30E Amplification Device.
Figure: The mScarlet-I gene was fused with relE gene to detect the amount of relE protein synthesis (579/616nm). Control group: pSB1C3-sulAp-relE-mSarlet-l-3WJxBro; experimental group: pSB1C3-sulAp-Amp30E-relE-mSarlet-l-3WJxBro.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 667
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None