Reporter
Part:BBa_K3977002
Designed by: Yilong Xu Group: iGEM21_SCU-China (2021-10-01)
mSarlet-I
mSarlet-I
Usage and Biology
Characterized by CAFA_China 2022
- The mScarlet-I gene and the relE toxin gene were fused to construct a fusion gene, in order to detect the amount of relE protein synthesis. We removed the terminator of relE and fused it to the mScarlet-I gene.
- We designed the genetic circuit that contains sulAp (sulA promoter), Amp30E, relE gene (RelE toxin), mScarlet-I and 3WJ-Bro(3WJ-Bro) , and transferred it into DH10B competent cells (The control group did not contain the Amp30E Amplification Device). The diagram is shown bellow:
- After bacterial culture and UV induction, we measured the OD600 and fluorescence values under 579/616nm. Then, the fluorescence per OD was calculated.
- The curve of experimental group was higher than that of control group as shown in the following figure, indicating the mScarlet-I correctly displayed the increasing tendency of relE protein synthesis under the effect of Amp30E Amplification Device.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 667
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |