Coding
Part:BBa_K4257000:Design
Designed by: Xiaohui Ma Group: iGEM22_CPU_Nanjing (2022-09-03)
PPK-M
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
To test whether the synthesized polyP can be visualized intracellularly, this part will be over-expressed in E.coli K12 and the engineered strain will be subjected to microscopic examination after staining.
Source
The EcPPK coding sequence was obtained by PCR amplification using the genomic DNA of E.coli K12 as the template.