Coding

Part:BBa_K4257000:Design

Designed by: Xiaohui Ma   Group: iGEM22_CPU_Nanjing   (2022-09-03)
Revision as of 06:31, 3 September 2022 by Registry (Talk | contribs)

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PPK-M


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To test whether the synthesized polyP can be visualized intracellularly, this part will be over-expressed in E.coli K12 and the engineered strain will be subjected to microscopic examination after staining.


Source

The EcPPK coding sequence was obtained by PCR amplification using the genomic DNA of E.coli K12 as the template.

References