Coding

Part:BBa_K4257000:Design

Designed by: Xiaohui Ma   Group: iGEM22_CPU_Nanjing   (2022-09-03)


PPK-M


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Highly active version of E. coli native PPK.

Source

The PPK-M coding sequence was obtained by PCR-mediated site-directed mutagenesis using the genomic DNA of E.coli K12 as the template.

References

Rudat, A. K., et al. (2018). "Mutations in Escherichia coli polyphosphate kinase that lead to dramatically increased in vivo polyphosphate levels." 200(6): e00697-00617.