Composite

Part:BBa_K4421030

Designed by: Shuyang Zhu   Group: iGEM22_NMU_China   (2022-07-15)
Revision as of 06:58, 16 July 2022 by Juejue (Talk | contribs)


pSV40-UAS-iCASP9-2A-GFP

An inducible caspase-9 suicide gene was placed under the control of a combined UAS-SV40 promoter. The GFP tag serves as a reporter and the 2A peptide sequence was intercalated between iCASP9 and the GFP tag to allow the fusion peptide chain to separate into two peptide chains.

  • pSV40-UAS-iCASP9-2A-GFP

The mechanism and usage of pSV40-UAS-iCASP9-2A-GFP

This part is placed downstream of NFAT_RE-Gal4-KRAB(BBa_K4421029) and the presence of Gal4-KRAB can inhibit the promotor pSV40-UAS(BBa_K4040003), thus diminishing the synthesis of the iCASP9(BBa_K4421002).The engineered cells can survive if the concentration of iCASP9 is low enough after AP1903 addition.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 781
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 463
    Illegal BsaI.rc site found at 1603
    Illegal BsaI.rc site found at 2514


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