Coding

Part:BBa_K3738032:Design

Designed by: Emily Hagens and Mark Lea   Group: iGEM21_Lethbridge   (2021-10-21)
Revision as of 00:27, 22 October 2021 by Registry (Talk | contribs)

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MlrA and C-Terminal and Anionic Tag (Basic)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 166
    Illegal BglII site found at 907
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 849


Design Notes

We have added an anionic tag for MS2-encapsulation


Source

Source: MlrA from Sphingopyxis sp. USTB-05

References