Coding

Part:BBa_K3738032:Design

Designed by: Emily Hagens and Mark Lea   Group: iGEM21_Lethbridge   (2021-10-21)


MlrA and C-Terminal and Anionic Tag (Basic)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 166
    Illegal BglII site found at 907
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 849


Design Notes

We have added an anionic tag for MS2-encapsulation


Source

Source: MlrA from Sphingopyxis sp. USTB-05

References

Saito T, Okano K, Park H-D, Itayama T, Inamori Y, Neilan BA, et al. Detection and sequencing of the microcystin LR-degrading gene, mlrA, from new bacteria isolated from Japanese lakes. FEMS Microbiology Letters. 2003;229(2):271-6. doi: 10.1016/S0378-1097(03)00847-4.

Jiang Y, Shao J, Wu X, Xu Y, Li R. Active and silent members in the mlr gene cluster of a microcystin-degrading bacterium isolated from Lake Taihu, China. FEMS Microbiology Letters. 2011;322(2):108-14. doi: 10.1111/j.1574-6968.2011.02337.x.

Wei J, Huang F, Feng H, Massey IY, Clara T, Long D, et al. Characterization and Mechanism of Linearized-Microcystinase Involved in Bacterial Degradation of Microcystins. Frontiers in Microbiology. 2021;12(527). doi: 10.3389/fmicb.2021.646084.