Composite

Part:BBa_K3889101

Designed by: Prameya Garge, Ashwin Sharma   Group: iGEM21_IISER-Tirupati_India   (2021-10-08)
Revision as of 12:19, 19 October 2021 by AshWinShaRma (Talk | contribs)


P22 expression cassette Bacillus subtilis

BBa_K3889150 contains SRTF1 binding site (BBa_K3889030) allowing expression of P22 c2 repressor (BBa_K3889020) only in the presence of progesterone if SRTF1 (BBa_K3889021) is being produced constitutively.

Azurite was linked with the help of P2A linker (BBa_K3889069)in order to coexpress it with P22 c2 repressor and check protein expression

Usage and Biology

This part along with BBa_K3889100 and BBa_K3889102 was used in order to produce Ovastacin (BBa_K3889022) when progesterone in absent in the fallopian tube. The working of the system is shown below:

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None