Coding
Part:BBa_K3712002
Designed by: Sheng Wang Group: iGEM21_HUST2-China (2021-10-19)
Optimized BLP-7
Although, BLP-7 has a huge power against bacteria, its antibacterial and anti-inflammatory effects are still able to be further improved. Theoretically, increasing the net positive charge and hydrophobicity of antimicrobial peptides can improve its antibacterial ability. In this project, we carried out amino acid mutation of wild-type BLP-7 through modeling design, replaced specific amino acids to improve its hydrophobicity, so that antimicrobial peptides could better bind to bacterial cell membranes and improve its drilling ability. In this way, we expect to obtain a more stable mutant BLP-7 with better antibacterial and anti-inflammatory effects.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 26
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 26
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 26
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 26
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
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