Part:BBa_K3712002

Optimized BLP-7

USAGE AND BIOLOGY

Although, BLP-7 has a huge power against bacteria, its antibacterial and anti-inflammatory effects are still able to be further improved. Theoretically, increasing the net positive charge and hydrophobicity of antimicrobial peptides can improve its antibacterial ability. In this project, we carried out amino acid mutation of wild-type BLP-7 through modeling design, replaced specific amino acids to improve its hydrophobicity, so that antimicrobial peptides could better bind to bacterial cell membranes and improve its drilling ability. In this way, we expect to obtain a more stable mutant BLP-7 with better antibacterial and anti-inflammatory effects.

RESULTS

We carry out antibacterial experiments respectively with purified optimized BLP-7-27 ELP. Firstly, we build an anaerobic environment for Propionibacterium acnes with anaerobic bag indicator and hermetic bag and cultured at 180 rpm, 37℃. Then we use the inhibition zone method and absorbance method to measure the bacteriostatic ability of the antimicrobial peptides. Unfortunately, due to the preliminary exploration of anaerobic culture and lack of time, we fail to measure the minimum bacteriostatic concentration of the antimicrobial peptides. We will explore the conditions of bacteriostatic experiments in the future.

FUTURE DIRECTIONS

Due to the lack of experience and method of Propionibacterium acnes cultivation, we fail to ascertain the optimal growth condition of Propionibacterium acnes after a long period of exploration although we successfully culture it. Subsequent exploration will be carried out to find the appropriate method to control the growth of Propionibacterium acnes.After determining the optimal growth conditions, we will further explore the principles of antibacterial experiments due to the uncertainty of incubation time and temperature of antimicrobial peptides.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 26
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 26
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 26
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 26
  
• 1000
  COMPATIBLE WITH RFC[1000]